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Biuret reagent is used for measuring total protein concentration. It comprises of copper II (cupric ion) alkaline solution, which interacts with the peptide bond to form a blue adduct which is measured at 540nm.
A biuret method has been developed which provides quantitative measurements of protein in normal urine without interference from drugs or pigments. This method is intended for use in monitoring clinical trials of new drugs to detect nephrotoxicity.
In this respect it is a less reliable assay than the biuret method, High-dose penicillin-G causes negative interference in the BCP method [10].
Biuret Reagent 110 ml (Product Code B 3934) Reagent contains 0.75 mM cupric sulfate and 94 mM sodium hydroxide. It also contains tartrate, iodide, and carbonate.