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Silica Gel 100-200 Mesh grade of Silica Gel is mostly suitable for gravitational chromatography. This particle size also allows the solvent to flow smoothly without any pressure. Best for a compound that can be easily separated.
Silica gel high-purity grade, pore size 60 Å, 230-400 mesh particle size, 40-63 μm particle size, for flash chromatography | 112926-00-8. Applications Products Services Support.
Silica gel and alumina are highly polar so they interact strongly with polar compounds and solvents, and weakly with nonpolar molecules. The stationary phase is loaded into the column as a slurry with the solvent and is then packed by flowing solvent through the stationary phase.
Choice of column size is less important than the amount of silica used. However, in practice, most people will choose a column size that allows them to fill column approximately one-third to a half full of silica – not including the solvent reservoir (Fig. 6).