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Leishman stain is used in microscopy for staining blood smears. It provides excellent stain quality. It is generally used to differentiate and identify leucocytes, malaria parasites, and trypanosoma. It is based on a methanolic mixture of "polychromed" methylene blue.
It consists of a mixture of eosin (an acidic stain), and methylene blue (a basic stain) in alcohol and is usually diluted and buffered before use. It stains the different components of blood in a range of shades between red and blue. The similar Wright's stain is favoured by American workers.
Atlas Leishman's stain is used for staining of blood and bone marrow. It is generally used to differentiate and identify leucocytes, malaria parasites, and trypanosomas (unicellar parasitic protozoa). It is based on a mixture of methylene blue and eosin.