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Methyl Violet 10B powder dye is intended for routine staining according to Gram, for staining bacterial components and for staining amyloids. It is often used for polychromatic section staining in epoxy resins, for viability neuron staining in cell culture, and for meiotic structures analysis.
The main use of methyl violet is to dye textiles purple and give deep violet and blue colors in paints and printing ink. Methyl violet 2B (simply called methyl violet) is used in chemistry as a pH indicator. Methyl violets are mixtures of tetramethyl, pentamethyl and hexamethyl pararosanilins.
Crystal violet has an antibacterial action against Escherichia coli, Staphylococcus aureus, Streptococcus faecalis and Bacillus subtilis. The effect of the dye, measured as minimum inhibitory concentration or retardation of growth, increases as the pH rises from 6 to 8. ... Gram-negative organisms, such as E.
In the Gram staining method, crystal violet is used to differentiate between Gram Positive and Gram Negative bacteria.